Proteintech, 28494-1-AP, CRBN Polyclonal antibody

Size: 20ul / 150ul The CRBN (28494-1-AP) by Proteintech is a Polyclonal antibody targeting CRBN in WB, IHC, IF/ICC, ELISA applications with reactivity to human, mouse, rat samples 28494-1-AP targets CRBN in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: 3T3-L1 cells, COLO 320 cells, mouse liver tissue, U-251 cells, C6 cells Positive IHC detected in: mouse brain tissue, human liver cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: COLO 320 cells Recommended dilution Western Blot (WB): WB : 1:1000-1:8000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Background Information CRBN plays an important role in assembly and surface expression of functional BK(Ca) channels. The mutation of human cereblon gene (CRBN) is related with mild mental retardation, and causes ARNSID (nonsyndromal intellectual disability) by distubing the developmental regulation of BK(Ca) in brain regions that are critical for memory and learning. CRBN is also identified as important thalidomide-binding protein for limb outgrowth in zebrafish and chicks. It can be detected a band above 51 kDa (maybe 55 kDa) in HT22 cells(PMID: 21232561). Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag29353 Product name: Recombinant human CRBN protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-181 aa of BC017419 Sequence: MAGEGDQQDAAHNMGNHLPLLPAESEEEDEMEVEDQDSKEAKKPNIINFDTSLPTSHTYLGADMEEFHGRTLHDDDSCQVIPVLPQVMMILIPGQTLPLQLFHPQEVSMVRNLIQKDRTFAVLAYSNVQEREAQFGTTAEIYAYREEQDFGIEIVKVKAIGRQRFKVLELRTQSDGIQQAK Predict reactive species Full Name: cereblon Calculated Molecular Weight: 442 aa, 51 kDa Observed Molecular Weight: 51 kDa GenBank Accession Number: BC017419 Gene Symbol: CRBN Gene ID (NCBI): 51185 RRID: AB_2881155 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q96SW2 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.