Proteintech, 28513-1-AP, TYRO3 Polyclonal antibody

Size: 20ul / 150ul The TYRO3 (28513-1-AP) by Proteintech is a Polyclonal antibody targeting TYRO3 in WB, IHC, IF/ICC, ELISA applications with reactivity to human, mouse, rat samples 28513-1-AP targets TYRO3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: mouse brain tissue, rat brain tissue Positive IHC detected in: human colon cancer tissue, mouse brain tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HCT 116 cells Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information TYRO3 has a structure includes a cytoplasmic domain and an extracellular ligand binding region. And ligand binding at the cell surface induces dimerization and autophosphorylation of TYRO3 on its intracellular domain that provides docking sites for downstream signaling molecules. TYRO3 is associated with many physiological processes including cell survival, migration and differentiation. It has been reported that TYRO3 can be expressed in various regions of the mammalian central nervous system. 28513-1-AP antibody recognizes the 97 kDa and 140 kDa protein which might be the glycosylated form similar to the papers.((PMID: 8873131, 23354874, 21539887) Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag29540 Product name: Recombinant human TYRO3 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 451-596 aa of BC049368 Sequence: LRKRRKETRFGQAFDSVMARGEPAVHFRAARSFNRERPERIEATLDSLGISDELKEKLEDVLIPEQQFTLGRMLGKGEFGSVREAQLKQEDSSFVKVAVKMLKADIIASSDIEEFLREAACMKEFDHPHVAKLVGVSLRSRAKGRL Predict reactive species Full Name: TYRO3 protein tyrosine kinase Calculated Molecular Weight: 97 kDa Observed Molecular Weight: 140 kDa GenBank Accession Number: BC049368 Gene Symbol: TYRO3 Gene ID (NCBI): 7301 RRID: AB_2881162 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q06418 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.