Proteintech, 28618-1-AP, VPS13A Polyclonal antibody

Size: 20ul / 150ul The VPS13A (28618-1-AP) by Proteintech is a Polyclonal antibody targeting VPS13A in WB, IHC, ELISA applications with reactivity to human, mouse samples 28618-1-AP targets VPS13A in WB, IHC, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: HeLa cells, mouse brain tissue, mouse heart tissue Positive IHC detected in: mouse brain tissue, human kidney tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:1000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information VPS13 (vacuolar protein sorting 13) acts at membrane contact sites between intracellular organelles to transport lipids. There are four VPS13 family members in human - VPS13A, VPS13B, VPS13C and VPS13D. Mutations in human VPS13 genes are linked to rare neurodegenerative disorders: chorea- acanthocytosis (VPS13A), Cohen syndrome (VPS13B), predispose to early onset into Parkinson disease (VPS13C) and lead to ataxia/spastic paraplegia (VPS13D) (PMID: 30656912). Specification Tested Reactivity: human, mouse Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag29532 Product name: Recombinant human VPS13A protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 2452-2595 aa of NM_033305 Sequence: PVGSRRLKWRCRKSHGEVTQKDDMMMPIDLGEKTIYLVSFFEGLQRIILFTEDPRVFKVTYESEKAELAEQEIAVALQDVGISLVNNYTKQEVAYIGITSSDVVWETKPKKKARWKPMSVKHTEKLEREFKEYTESSPSEDKVI Predict reactive species Full Name: vacuolar protein sorting 13 homolog A (S. cerevisiae) Calculated Molecular Weight: 360 kDa Observed Molecular Weight: 360 kDa GenBank Accession Number: NM_033305 Gene Symbol: VPS13A Gene ID (NCBI): 23230 RRID: AB_2918183 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q96RL7 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.