Proteintech, 28686-1-AP, Ajuba Polyclonal antibody

Size: 20ul / 150ul The Ajuba (28686-1-AP) by Proteintech is a Polyclonal antibody targeting Ajuba in WB, IHC, ELISA applications with reactivity to Human, mouse, rat samples 28686-1-AP targets Ajuba in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples. Tested Applications Positive WB detected in: HeLa cells, HepG2 cells Positive IHC detected in: mouse skin tissue, human thyroid cancer tissue, mouse small intestine tissue, mouse testis tissue, rat skin tissue, rat small intestine tissue, rat testis tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:1000-1:6000 Immunohistochemistry (IHC): IHC : 1:500-1:2000 Background Information Ajuba belongs to the LIM domain proteins, which are involved in the assembly of the extracellular matrix and, along with associated proteins, regulate target genes that connect the extracellular matrix and the cytoskeleton. Ajuba consists of three LIM domains at the C-terminal and is located in the cytoplasm where it interacts with a variety of other proteins. Ajuba contributes to the formation or strengthening of cell-cell adhesion and regulates cell growth and differentiation decisions. Ajuba participates in the assembly of the extracellular matrix. Ajuba also plays an important role in some diseases. Specification Tested Reactivity: Human, mouse, rat Cited Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag29725 Product name: Recombinant human JUB protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-173 aa of BC007580 Sequence: MERLGEKASRLLEKFGRRKGESSRSGSDGTPGPGKGRLSGLGGPRKSGPRGATGGPGDEPLEPAREQGSLDAERNQRGSFEAPRYEGSFPAGPPPTRALPLPQSLPPDFRLEPTAPALSPRSSFASSSASDASKPSSPRGSLLLDGAGAGGAGGSRPCSNRTSGISMGYDQRH Predict reactive species Full Name: jub, ajuba homolog (Xenopus laevis) Calculated Molecular Weight: 57 kDa Observed Molecular Weight: 57 kDa GenBank Accession Number: BC007580 Gene Symbol: Ajuba Gene ID (NCBI): 84962 RRID: AB_2881194 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q96IF1 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.