Proteintech, 28703-1-AP, MUC4 Polyclonal antibody

Size: 20ul / 150ul The MUC4 (28703-1-AP) by Proteintech is a Polyclonal antibody targeting MUC4 in WB, IHC, ELISA applications with reactivity to human samples 28703-1-AP targets MUC4 in WB, IHC, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: HeLa cells, MCF-7 cells Positive IHC detected in: human prostate cancer tissue, human breast cancer tissue, human colon tissue, human colon cancer tissue, human lung cancer tissue, human pancreas cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:3000 Immunohistochemistry (IHC): IHC : 1:200-1:1000 Background Information MUC4, also named as HSA276359, ASGP, MUC-4, testis mucin and mucin-4 is a high molecular weight transmembrane mucin that is overexpressed in many carcinomas and is a risk factor associated with a poor prognosis. MUC4 have a key role in the progression of pancreatic cancer. MUC4 helps in the docking of tumor cells on the endothelial surface. Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag29799 Product name: Recombinant human MUC4 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 29-225 aa of NM_018406 Sequence: TTEDTLITGSKTAAPVTSTGSTTATLEGQSTAASSRTSNQDISASSQNHQTKSTETTSKAQTDTLTQMMTSTLFSSPSVHNVMETVTQETAPPDEMTTSFPSSVTNTLMMTSKTITMTTSTDSTLGNTEETSTAGTESSTPVTSAVSITAGQEGQSRTTSWRTSIQDTSASSQNHWTRSTQTTRESQTSTLTHRTTS Predict reactive species Full Name: mucin 4, cell surface associated Calculated Molecular Weight: 231 kDa Observed Molecular Weight: 231 kDa, 113 kDa GenBank Accession Number: NM_018406 Gene Symbol: MUC4 Gene ID (NCBI): 4585 RRID: AB_2918192 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q99102 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.