Proteintech, 28733-1-AP, Phospho-ERK1/2 (Thr202/Tyr204) Polyclonal antibody

Size: 20ul / 100ul The Phospho-ERK1/2 (Thr202/Tyr204) (28733-1-AP) by Proteintech is a Polyclonal antibody targeting Phospho-ERK1/2 (Thr202/Tyr204) in WB, IP, ELISA applications with reactivity to human, mouse samples 28733-1-AP targets Phospho-ERK1/2 (Thr202/Tyr204) in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: NIH/3T3 cells, Calyculin A treated HEK-293T cells Positive IP detected in: Calyculin A treated PC-3 cells Recommended dilution Western Blot (WB): WB : 1:1000-1:4000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Background Information Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. MAPK1/ERK2 and MAPK3/ERK1 are the 2 MAPKs which play an important role in the MAPK/ERK cascade. They participate also in a signaling cascade initiated by activated KIT and KITLG/SCF. Depending on the cellular context, the MAPK/ERK cascade mediates diverse biological functions such as cell growth, adhesion, survival and differentiation through the regulation of transcription, translation, cytoskeletal rearrangements. The MAPK/ERK cascade plays also a role in initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors. MEK1 and MEK2 activate p44 and p42 through phosphorylation of activation loop residues Thr202/Tyr204 and Thr185/Tyr187, respectively. Several downstream targets of p44/42 have been identified, including p90RSK and the transcription factor Elk-1. This antibody simultaneously recognizes the phosphorylation of ERK1 at Thr202 and Tyr204, as well as the phosphorylation of ERK2 at Thr185 and Tyr187. Specification Tested Reactivity: human, mouse Cited Reactivity: human, mouse, rat, pig, chicken, zebrafish, sheep, goat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: mitogen-activated protein kinase 3 Calculated Molecular Weight: 38-43 kDa Observed Molecular Weight: 38-43 kDa GenBank Accession Number: NM_002746 Gene Symbol: ERK1 Gene ID (NCBI): 5595 RRID: AB_2881202 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P27361 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.