Proteintech, 28750-1-AP, PLK4 Polyclonal antibody

Size: 20ul / 150ul The PLK4 (28750-1-AP) by Proteintech is a Polyclonal antibody targeting PLK4 in IHC, IF/ICC, ELISA applications with reactivity to Human, Mouse samples 28750-1-AP targets PLK4 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, Mouse samples. Tested Applications Positive IHC detected in: human colon cancer tissue, mouse testis tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: hTERT-RPE1 cells Recommended dilution Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Background Information PLK4 (Polo-like kinase 4) is also named SAK, STK18 and belongs to the Ser/Thr protein kinase family. It plays a central role in centriole duplication and is a kinase implicated in tumor development, which is an upstream regulator of canonical biogenesis necessary for centriole formation. It has 3 isoforms produced by alternative splicing. Specification Tested Reactivity: Human, Mouse Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag30482 Product name: Recombinant human PLK4 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 235-426 aa of BC036023 Sequence: SIEAKDLIHQLLRRNPADRLSLSSVLDHPFMSRNSSTKSKDLGTVEDSIDSGHATISTAITASSSTSISGSLFDKRRLLIGQPLPNKMTVFPKNKSSTDFSSSGDGNSFYTQWGNQETSNSGRGRVIQDAEERPHSRYLRRAYSSDRSGTSNSQSQAKTYTMERCHSAEMLSVSKRSGGGENEERYSPTDNN Predict reactive species Full Name: polo-like kinase 4 (Drosophila) Calculated Molecular Weight: 970 aa, 109 kDa GenBank Accession Number: BC036023 Gene Symbol: PLK4 Gene ID (NCBI): 10733 RRID: AB_2881206 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: O00444 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.