Proteintech, 28792-1-AP, Phospho-CREB1 (Ser133) Polyclonal antibody

Size: 20ul / 100ul The Phospho-CREB1 (Ser133) (28792-1-AP) by Proteintech is a Polyclonal antibody targeting Phospho-CREB1 (Ser133) in WB, IHC, ELISA applications with reactivity to human, mouse, rat samples 28792-1-AP targets Phospho-CREB1 (Ser133) in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: Forskolin treated HSC-T6 cells, HeLa cells, NIH/3T3 cells, Forskolin treated NIH/3T3 cells Positive IHC detected in: human liver tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:1000-1:8000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information The cAMP-response element binding protein (CREB) is localized in the nucleus and acts as a transcription factor, which binds to the cAMP response element (CRE) of the promoters of its target genes, upon phosphorylation at Ser133 by different receptor-activated protein kinases, such as protein kinase A (PKA), calmodulin-dependent protein kinase (CaMK), mitogen-activated protein kinases (MAPK), and other kinases. The activity of CREB1 in neurons has been correlated with various intracellular processes, including proliferation, differentiation, survival, long-term synaptic potentiation, neurogenesis, and neuronal plasticity. CREB1 belongs to the CREB/activating transcription factor (ATF) family of transcription factors. (PMID: 30214393, PMID: 33917483, PMID: 32605164) The antibody also detects the phosphorylated form of the CREB-related protein ATF1. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: cAMP responsive element binding protein 1 Calculated Molecular Weight: 341 aa, 35 kDa Observed Molecular Weight: 35-46 kDa GenBank Accession Number: BC010636 Gene Symbol: CREB1 Gene ID (NCBI): 1385 RRID: AB_2918203 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P16220 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.