Proteintech, 28851-1-AP, Phospho-Neurofibromin 2 (Ser518) Polyclonal antibody

Size: 20ul / 100ul The Phospho-Neurofibromin 2 (Ser518) (28851-1-AP) by Proteintech is a Polyclonal antibody targeting Phospho-Neurofibromin 2 (Ser518) in WB, ELISA applications with reactivity to Human samples 28851-1-AP targets Phospho-Neurofibromin 2 (Ser518) in WB, IHC, ELISA applications and shows reactivity with Human samples. Tested Applications Positive WB detected in: Calyculin A treated HEK-293T cells, Calyculin A treated PC-3 cells, HEK-293T cells Recommended dilution Western Blot (WB): WB : 1:1000-1:8000 Background Information Neurofibromatosis 2 (NF2) tumor suppressor protein-merlin is involved in Hippo/SWH signaling pathway that associated with tumor suppression by restricting proliferation and promoting apoptosis. It has been reported that merlin can change to phosphorylation form which inactivates merlin tumor suppressive abilities at serine 518 induced by Rac effector p21-activated kinase 2. The mutation of the NF2 tumor suppressor gene may lead to nervous system tumors, mesotheliomas and metastatic tumors. 28851-1-AP antibody is specific to phosphorylation of NF2 at serine 518. (PMID: 11782491, 14724586, 18361411, 18835652, 32115406) Specification Tested Reactivity: Human Cited Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: neurofibromin 2 (merlin) Calculated Molecular Weight: 70 kDa Observed Molecular Weight: 70 kDa GenBank Accession Number: BC007279 Gene Symbol: NF2 Gene ID (NCBI): 4771 RRID: AB_2935410 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P35240 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.