Proteintech, 28915-1-AP, Phospho-c-MYC (Ser62) Polyclonal antibody

Size: 20ul / 100ul The Phospho-c-MYC (Ser62) (28915-1-AP) by Proteintech is a Polyclonal antibody targeting Phospho-c-MYC (Ser62) in WB, IF/ICC, ELISA applications with reactivity to human samples 28915-1-AP targets Phospho-c-MYC (Ser62) in WB, IF/ICC, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: MG132 treated HEK-293T cells, HEK-293T cells Positive IF/ICC detected in: λ phosphatase treated HeLa cells Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Background Information MYC is a transcription factor that globally enhances expression of transcribing genes, including those vital for cell cycle, growth, proliferation, and survival in normal and cancer cells. MYC has multiple isomers, and this antibody recognizes the phosphorylation of the 439 amino acid isomer (P01106-1, UniProt) at the 62 serine site. Posttranslational modifications that regulate MYC stability include phosphorylations at Ser62 and Thr58. Phosphorylation at Ser62, primarily by ERK, stabilizes MYC. While kinases other than ERK phosphorylate Ser62, GSK3β has been the only kinase known to phosphorylate MYC at Thr58.(PMID: 32482868) Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: v-myc myelocytomatosis viral oncogene homolog (avian) Calculated Molecular Weight: 49 kDa Observed Molecular Weight: 55 kDa GenBank Accession Number: BC000141 Gene Symbol: MYC Gene ID (NCBI): 4609 RRID: AB_3085340 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P01106 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.