Proteintech, 28939-1-AP, Phospho-eNOS (Thr495) Polyclonal antibody

Size: 20ul / 100ul The Phospho-eNOS (Thr495) (28939-1-AP) by Proteintech is a Polyclonal antibody targeting Phospho-eNOS (Thr495) in WB, ELISA applications with reactivity to human samples 28939-1-AP targets Phospho-eNOS (Thr495) in WB, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: VEGF treated HUVEC cells, EGF treated HUVEC cells, Cobalt Chloride treated HUVEC cells Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Background Information Endothelial NOS (eNOS), also known as nitric oxide synthase 3 (NOS3), has a protective function in the cardiovascular system, which is attributed to NO production. Polymorphisms in NOS3 gene affects the susceptibility to several diseases such as hypertension, preeclampsia, diabetes mellitus, obesity, erectile dysfunction, and migraine. The sequence homology of eNOS protein in human and mouse is above 90%, and phosphorylation of threonine at 495 site in human corresponds to 494 site in mouse. Specification Tested Reactivity: human Cited Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: nitric oxide synthase 3 (endothelial cell) Calculated Molecular Weight: 133 kDa Observed Molecular Weight: 140 kDa GenBank Accession Number: BC063294 Gene Symbol: eNOS Gene ID (NCBI): 4846 RRID: AB_2881234 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P29474 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.