Proteintech, 28965-1-AP, Phospho-STK11/LKB1 (Thr189) Polyclonal antibody

Size: 20ul / 100ul The Phospho-STK11/LKB1 (Thr189) (28965-1-AP) by Proteintech is a Polyclonal antibody targeting Phospho-STK11/LKB1 (Thr189) in WB, ELISA applications with reactivity to Human, mouse samples 28965-1-AP targets Phospho-STK11/LKB1 (Thr189) in WB, ELISA applications and shows reactivity with Human, mouse samples. Tested Applications Positive WB detected in: Calyculin A treated PC-3 cells, UV treated NIH/3T3 cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Background Information Liver kinase 1 (LKB1 also known as STK11) is a tumor suppressor gene that is inactivated by bi-allelic mutation in non-small cell lung cancer (NSCLC), malignant melanoma and cervical cancer. LKB1 protein has a central kinase domain, two N-terminal nuclear leading sequences, and a C-terminal regulatory motif. The protein also contains a CAAX motif at C-terminal end and can be farnesylated. The formation of an LKB1/Strad/MO25 complex is essential for LKB1's kinase activity, and there are at least 13 known substrates of LKB1, which includes AMPKs, BRSKs, and MARKs. LKB1 is the major kinase that phosphorylates the AMPK activation loop in conditions of energy stress. (PMID: 25679014, PMID: 19629071) Specification Tested Reactivity: Human, mouse Cited Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: serine/threonine kinase 11 Calculated Molecular Weight: 49 kDa Observed Molecular Weight: 50-55 kDa GenBank Accession Number: BC007981 Gene Symbol: LKB1 Gene ID (NCBI): 6794 RRID: AB_2918225 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q15831 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.