Proteintech, 29006-1-AP, Phospho-ULK1 (Thr468) Polyclonal antibody

Size: 20ul / 100ul The Phospho-ULK1 (Thr468) (29006-1-AP) by Proteintech is a Polyclonal antibody targeting Phospho-ULK1 (Thr468) in WB, IF/ICC, ELISA applications with reactivity to human samples 29006-1-AP targets Phospho-ULK1 (Thr468) in WB, IF/ICC, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: λ phosphatase treated HeLa cells Positive IF/ICC detected in: HeLa cells Recommended dilution Western Blot (WB): WB : 1:1000-1:8000 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Background Information Unc-51-like-kinase 1 (ULK1) is a target of both the mechanistic target of rapamycin (mTOR) and AMP activated protein kinase (AMPK), whose role is to facilitate the initiation of autophagy in response to starvation. ULK1 is phosphorylated on serine 638 and 758 sites by mTOR in nutrient-rich conditions, inhibiting ULK1 activation by disrupting its binding to AMPK. Upon glucose starvation, dissociation of mTOR from ULK1 and phosphorylation by AMPK leads to the activation of ULK1 activity. S467, S556, and T574 are sites responsible for regulating mitochondrial homeostasis during starvation. (PMID: 30517873, PMID: 21258367) Specification Tested Reactivity: human Cited Reactivity: human, mouse, rat, pig Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: unc-51-like kinase 1 (C. elegans) Observed Molecular Weight: 130-140 kDa GenBank Accession Number: NM_003565 Gene Symbol: ULK1 Gene ID (NCBI): 8408 RRID: AB_2918229 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: O75385 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.