Proteintech, 29018-1-AP, Phospho-YAP1 (Ser397) Polyclonal antibody

Size: 20ul / 100ul The Phospho-YAP1 (Ser397) (29018-1-AP) by Proteintech is a Polyclonal antibody targeting Phospho-YAP1 (Ser397) in WB, ELISA applications with reactivity to human, mouse samples 29018-1-AP targets Phospho-YAP1 (Ser397) in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: Serum-free treated NIH/3T3 cells, 10 % fetal bovine serum treated NIH/3T3 cells Recommended dilution Western Blot (WB): WB : 1:2000-1:16000 Background Information The transcriptional factors Yes-associated protein 1 (YAP1) and PDZ-binding motif (TAZ) act as downstream effectors of the Hippo pathway, and their subcellular location and transcriptional activities are affected by multiple post-translational modifications (PTMs). After phosphorylation, YAP1/TAZ binds to the 14-3-3 protein, which induces cytoplasmic retention of YAP1/TAZ. Moreover, phosphorylated YAP1/TAZ is likely to undergo ubiquitination degradation that is dependent on the β-transducin repeat-containing E3 ubiquitin protein ligase complex. The phosphorylation of YAP at Ser127 promotes its cytoplasmic retention, whereas phosphorylation at Ser397 induces degradation. (PMID: 29682330, PMID: 26039999) Specification Tested Reactivity: human, mouse Cited Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: Yes-associated protein 1, 65kDa Calculated Molecular Weight: 504 aa, 54 kDa Observed Molecular Weight: 75 kDa GenBank Accession Number: BC038235 Gene Symbol: YAP1 Gene ID (NCBI): 10413 RRID: AB_2923587 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P46937 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.