Proteintech, 29250-1-AP, Phospho-MAP2K4 (Ser257/Thr261) Polyclonal antibody

Size: 20ul / 100ul The Phospho-MAP2K4 (Ser257/Thr261) (29250-1-AP) by Proteintech is a Polyclonal antibody targeting Phospho-MAP2K4 (Ser257/Thr261) in WB, ELISA applications with reactivity to Human samples 29250-1-AP targets Phospho-MAP2K4 (Ser257/Thr261) in WB, ELISA applications and shows reactivity with Human samples. Tested Applications Positive WB detected in: PDGF treated PC-3 cells Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Background Information Mitogen-activated protein kinase kinase 4 (MAP2K4), also known as MAP kinase kinase 4 (MKK4), is a second-level kinase in the hierarchical mitogen-activated protein kinase (MAPK) pathway and is regulated by Ras. MAP2K4 has been found to be stably mutated in 3-5 % of cancers, such as ovarian, prostate, and liver cancers. MAP2K4 is activated by MEKK1 or ASK1/TAK1/MLK3. It is unique among all known MAP2Ks in its ability to phosphorylate two different MAPKs, p38 and JNK (c-Jun N-terminal kinase), respectively. (PMID: 24487067, PMID: 33341545) Specification Tested Reactivity: Human Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: mitogen-activated protein kinase kinase 4 Calculated Molecular Weight: 399 aa, 44 kDa Observed Molecular Weight: 44-50 kDa GenBank Accession Number: BC036032 Gene Symbol: MAP2K4 Gene ID (NCBI): 6416 RRID: AB_2918258 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P45985 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.