Proteintech, 29285-1-AP, Nestin Polyclonal antibody

Size: 20ul / 150ul The Nestin (29285-1-AP) by Proteintech is a Polyclonal antibody targeting Nestin in WB, IF/ICC, IF-P, FC (Intra), ELISA applications with reactivity to human, mouse, rat samples 29285-1-AP targets Nestin in WB, IF/ICC, IF-P, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: SGC-7901 cells, U-251 cells, U-87 MG cells Positive IF-P detected in: rat brain tissue, human kidney tissue Positive IF/ICC detected in: U-251 cells Positive FC (Intra) detected in: SH-SY5Y cells Recommended dilution Western Blot (WB): WB : 1:500-1:1000 Immunofluorescence (IF)-P: IF-P : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension Background Information Nestin, also named as NES and Nbla00170, is a class VI intermediate filament protein expressed in stem cells of the central nervous system (CNS) but not in mature CNS cells. Its expression is used extensively as a marker for neural lineage cells derived from human embryonic stem (hES) and induced pluripotent stem (iPS) cells. Nestin is also expressed in non-neural stem cell populations such as pancreatic islet progenitors and hematopoietic progenitors. Nestin can be detected as 220-240 kDa and 240-280 kDa. Studies point toward nestin heavy posttranslational modifications as possibly serving particular functions in podocytes and in a few other particular cell types(PMID: 17210924). Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag29865 Product name: Recombinant human nestin protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1450-1621 aa of NM_006617 Sequence: SSSQRGEFLESDSVSVSVPWDDSLRGAVAGAPKTALETESQDSAEPSGSEEESDPVSLEREDKVPGPLEIPSGMEDAGPGADIIGVNGQGPNLEGKSQHVNGGVMNGLEQSEEVGQGMPLVSEGDRGSPFQEEEGSALKTSWAGAPVHLGQGQFLKFTQREGDRESWSSGED Predict reactive species Full Name: nestin Calculated Molecular Weight: 177 kDa Observed Molecular Weight: 240-280 kDa GenBank Accession Number: NM_006617 Gene Symbol: Nestin Gene ID (NCBI): 10763 RRID: AB_2918268 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P48681 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.