Proteintech, 29394-1-AP, TPBG Polyclonal antibody

Size: 20ul / 150ul The TPBG (29394-1-AP) by Proteintech is a Polyclonal antibody targeting TPBG in WB, IHC, ELISA applications with reactivity to Human, rat, mouse samples 29394-1-AP targets TPBG in WB, IHC, ELISA applications and shows reactivity with Human, rat, mouse samples. Tested Applications Positive WB detected in: HEK-293T cells, MCF-7 cells, mouse brain tissue, rat brain tissue Positive IHC detected in: mouse eye tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:1000-1:4000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information Trophoblast glycoprotein (TPBG), also termed 5T4, is a 72 kDa transmembrane glycoprotein and an extensively investigated oncofetal antigen, which is limited in normal adult tissues, but highly expressed in various types of cancer in human. TPBG is a novel PKCa-dependent phosphoprotein in retinal rod bipolar cells (RBCs). (PMID: 18097562, PMID: 31255707) Specification Tested Reactivity: Human, rat, mouse Cited Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag30019 Product name: Recombinant human TPBG protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 153-355 aa of NM_006670 Sequence: PLADLSPFAFSGSNASVSAPSPLVELILNHIVPPEDERQNRSFEGMVVAALLAGRALQGLRRLELASNHFLYLPRDVLAQLPSLRHLDLSNNSLVSLTYVSFRNLTHLESLHLEDNALKVLHNGTLAELQGLPHIRVFLDNNPWVCDCHMADMVTWLKETEVVQGKDRLTCAYPEKMRNRVLLELNSADLDCDPILPPSLQTS Predict reactive species Full Name: trophoblast glycoprotein Calculated Molecular Weight: 46 kDa Observed Molecular Weight: 72-80 kDa GenBank Accession Number: NM_006670 Gene Symbol: TPBG Gene ID (NCBI): 7162 RRID: AB_2918290 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q13641 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.