Proteintech, 29461-1-AP, SNF2L Polyclonal antibody

Size: 20ul / 150ul The SNF2L (29461-1-AP) by Proteintech is a Polyclonal antibody targeting SNF2L in WB, IHC, ELISA applications with reactivity to Human, mouse, rat samples 29461-1-AP targets SNF2L in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples. Tested Applications Positive WB detected in: U-87 MG cells, U-251 cells, SH-SY5Y cells Positive IHC detected in: mouse lung tissue, rat lung tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:2000-1:12000 Immunohistochemistry (IHC): IHC : 1:1000-1:4000 Background Information SNF2L, also named as SMARCA1, NURF140 and ISWI, encodes the SWI/SNF family of proteins. The encoded protein is an ATPase which is expressed in diverse tissues and contributes to the chromatin remodeling complex that is involved in transcription (PMID: 28801535). SNF2L was shown to promote neurite outgrowth as well as regulate the human engrailed homeotic genes, important regulators of brain development (PMID: 15310751). The protein may also play a role in DNA damage, growth inhibition and apoptosis of cancer cells (PMID: 25462860). SNF2L has 2 isoforms with MW of 123 kDa and 121 kDa, and can be detected as 120-130 kDa. Specification Tested Reactivity: Human, mouse, rat Cited Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag30657 Product name: Recombinant human SNF2L protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-90 aa of NM_003069 Sequence: MEQDTAAVAATVAAADATATIVVIEDEQPGPSTSQEEGAAAAATEATAATEKGEKKKEKNVSSFQLKLAAKAPKSEKEMDPEYEEKMKAD Predict reactive species Full Name: SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 1 Calculated Molecular Weight: 123 kDa Observed Molecular Weight: 120-130 KDa GenBank Accession Number: NM_003069 Gene Symbol: SMARCA1/SNF2L Gene ID (NCBI): 6594 RRID: AB_2918310 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P28370 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.