Proteintech, 29546-1-AP, Phospho-PERK/EIF2AK3 (Ser719) Polyclonal antibody

Size: 20ul / 100ul The Phospho-PERK/EIF2AK3 (Ser719) (29546-1-AP) by Proteintech is a Polyclonal antibody targeting Phospho-PERK/EIF2AK3 (Ser719) in WB, ELISA applications with reactivity to human samples 29546-1-AP targets Phospho-PERK/EIF2AK3 (Ser719) in WB, IHC, IF, IP, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: λ phosphatase treated HeLa cells, Thapsigargin treated HeLa cells Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Background Information EIF2AK3 encodes the protein kinase RNA-like ER kinase (PERK), a key regulator of the unfolded protein response (UPR) in response to ER stress. Under ER stress conditions, activation of PERK is triggered by the dissociation of glucose-regulated protein (GRP) 78 (also known as BiP) from its luminal domain, followed by oligomerization and autophosphorylation. Phosphorylated PERK subsequently phosphorylates eukaryotic translation initiation factor 2 alpha (eif2α), to attenuate global protein translation and reduce incoming ER protein load via upregulated ER chaperone expression. (PMID: 35922637, PMID: 32029570) Specification Tested Reactivity: human Cited Reactivity: human, mouse, rat, zebrafish, bovine Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: eukaryotic translation initiation factor 2-alpha kinase 3 Calculated Molecular Weight: 1116 aa, 125 kDa Observed Molecular Weight: 140 kDa GenBank Accession Number: BC126354 Gene Symbol: PERK Gene ID (NCBI): 9451 RRID: AB_2935416 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9NZJ5 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.