Proteintech, 29560-1-AP, Phospho-MCL1 (Thr163) Polyclonal antibody

Size: 20ul / 100ul The Phospho-MCL1 (Thr163) (29560-1-AP) by Proteintech is a Polyclonal antibody targeting Phospho-MCL1 (Thr163) in WB, ELISA applications with reactivity to Human samples 29560-1-AP targets Phospho-MCL1 (Thr163) in WB, ELISA applications and shows reactivity with Human samples. Tested Applications Positive WB detected in: MG132 treated HeLa cells Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Background Information MCL1 is an anti-apoptotic member of the BCL-2 family originally isolated from the ML-1 human myeloid leukemia cell line. Similar to BCL2 and BCL2L1, MCL1 can interact with BAX and/or BAK1 to inhibit mitochondria-mediated apoptosis. Recent studies show that MCL1 is upregulated in numerous hematological and solid tumor malignancies. Therefore, MCL1 has been suggested as a potential new therapeutic target. MCL1 can be phosphorylated by several protein kinases which enables the recognition of MCL1 by its E3 ubiquitin-ligases TrCP or FBW7 (PMID: 33308268). MCL1 shows higher stability when phosphorylated on threonine 163 (PMID: 16543145). Specification Tested Reactivity: Human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: myeloid cell leukemia sequence 1 (BCL2-related) Calculated Molecular Weight: 350 aa, 37 kDa Observed Molecular Weight: 40 kDa GenBank Accession Number: BC017197 Gene Symbol: MCL1 Gene ID (NCBI): 4170 RRID: AB_2935417 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q07820 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.