Proteintech, 29580-1-AP, Phospho-PDH E1 Alpha (Ser293) Polyclonal antibody

Size: 20ul / 100ul The Phospho-PDH E1 Alpha (Ser293) (29580-1-AP) by Proteintech is a Polyclonal antibody targeting Phospho-PDH E1 Alpha (Ser293) in WB, ELISA applications with reactivity to human samples 29580-1-AP targets Phospho-PDH E1 Alpha (Ser293) in WB, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: HeLa cells, λ phosphatase treated HeLa cells Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Background Information PDHA1(Pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial) is also named as PHE1A. Multiple post-translational modifications (PTMs) of PDHA1 have been identified, such as acetylation, succinylation, and tyrosine phosphorylation. However, most of these PTMs ultimately affect PDHA1 activity through their influence on the phosphorylation of Ser293, Ser232, and Ser300 which are regulated by PDKs(pyruvate dehydrogenase kinases) and PDPs(pyruvate dehydrogenase phosphatases).(PMID: 34667275) Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: pyruvate dehydrogenase (lipoamide) alpha 1 Calculated Molecular Weight: 43 kDa Observed Molecular Weight: 40 kDa GenBank Accession Number: BC002406 Gene Symbol: PDHA1 Gene ID (NCBI): 5160 RRID: AB_3669690 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P08559 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.