Proteintech, 29582-1-AP, Phospho-PDH E1 Alpha (Ser232) Polyclonal antibody

Size: 20ul / 100ul The Phospho-PDH E1 Alpha (Ser232) (29582-1-AP) by Proteintech is a Polyclonal antibody targeting Phospho-PDH E1 Alpha (Ser232) in WB, IF/ICC, ELISA applications with reactivity to Human samples 29582-1-AP targets Phospho-PDH E1 Alpha (Ser232) in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human samples. Tested Applications Positive WB detected in: HeLa cells, Serum-free treated HeLa cells, 10 % fetal bovine serum treated HeLa cells Positive IF/ICC detected in: λ phosphatase treated HepG2 cells Recommended dilution Western Blot (WB): WB : 1:1000-1:8000 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Background Information PDH E1 Alpha (PDHA1), as the major component of PDH, can be phosphorylated and inactivated by PDHK1. PDHA1 contains three serine residues (Ser232, Ser293, or Ser300) that can be reversibly phosphorylated by a dedicated family of four inhibitory pyruvate dehydrogenase kinases (PDHK1-4) and two reactivating phosphatases (PDP1, 2). Hypoxia induces the expression of PDHK1 and PDHK3 and hyperphosphorylates PDHA1. Phosphorylated PDHA1 inactivates the whole pyruvate dehydrogenase complex (PDC), reduces pyruvate entering into the tricarboxylic acid (TCA) cycle for oxidative phosphorylation, enhances the Warburg effect and promotes tumorigenesis. (PMID: 30993888, PMID: 34749809) Specification Tested Reactivity: Human Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: pyruvate dehydrogenase (lipoamide) alpha 1 Calculated Molecular Weight: 43 kDa Observed Molecular Weight: 40 kDa GenBank Accession Number: BC002406 Gene Symbol: PDHA1 Gene ID (NCBI): 5160 RRID: AB_2918327 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P08559 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.