Proteintech, 29781-1-AP, SCPEP1 Polyclonal antibody

Size: 20ul / 150ul The SCPEP1 (29781-1-AP) by Proteintech is a Polyclonal antibody targeting SCPEP1 in WB, IHC, ELISA applications with reactivity to Human, mouse samples 29781-1-AP targets SCPEP1 in WB, IHC, ELISA applications and shows reactivity with Human, mouse samples. Tested Applications Positive WB detected in: A431 cells Positive IHC detected in: mouse embryo tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:1000-1:4000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information SCPEP1 encodes retinoid-inducible serine carboxypeptidase (PMID:12975309). SCPEP1 and Cathepsin A play roles in regulation of vasoconstriction via inactivation of endothelin-1 (PubMed:24586188). The protein is widely expressed during mouse embryogenesis by immunohistochemical (PubMed:16461364). Scpep1 is proteolytically cleaved to a mature enzyme which is smaller than 51 kDa (PMID:16461364). Specification Tested Reactivity: Human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag31396 Product name: Recombinant human SCPEP1 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 221-452 aa of BC072405 Sequence: LLEDKGLAEVSKVAEQVLNAVNKGLYREATELWGKAEMIIEQNTDGVNFYNILTKSTPTSTMESSLEFTQSHLVCLCQRHVRHLQRDALSQLMNGPIRKKLKIIPEDQSWGGQATNVFVNMEEDFMKPVISIVDELLEAGINVTVYNGQLDLIVDTMGQEAWVRKLKWPELPKFSQLKWKALYSDPKSLETSAFVKSYKNLAFYWILKAGHMVPSDQGDMALKMMRLVTQQE Predict reactive species Full Name: serine carboxypeptidase 1 Calculated Molecular Weight: 452 aa, 51 kDa Observed Molecular Weight: 33-51 kDa GenBank Accession Number: BC072405 Gene Symbol: SCPEP1 Gene ID (NCBI): 59342 RRID: AB_2923608 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9HB40 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.