Proteintech, 29786-1-AP, NSUN4 Polyclonal antibody

Size: 20ul / 150ul The NSUN4 (29786-1-AP) by Proteintech is a Polyclonal antibody targeting NSUN4 in WB, IHC, ELISA applications with reactivity to human samples 29786-1-AP targets NSUN4 in WB, IHC, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: HepG2 cells, K-562 cells, SH-SY5Y cells Positive IHC detected in: human intrahepatic cholangiocarcinoma tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:1000-1:6000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information NSUN4 belongs to a family of RNA 5-methylcytosine (m5C) methyltransferases (MTases). MTERF4 recruits NSUN4 to the large ribosomal subunit in a process crucial for mitochondrial ribosome biogenesis. NSUN4 is a dual function protein, which on the one hand is needed for 12S rRNA methylation, and on the other hand interacts with MTERF4 to facilitate mitoribosomal assembly. The calculated MW of NSUN4 is 43 kDa, 29786-1-AP can detect the band around 40 kDa.(PMID:22949673, 24516400) Specification Tested Reactivity: human Cited Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag31821 Product name: Recombinant human NSUN4 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 26-284 aa of BC014441 Sequence: RYKKKWAATEPKFPAVRLALQNFDMAYSVQFGDLWPSIRVSLLSEQKYGALVNNFAAWDHVSAKLEQLSAKDFVNEAISHWELQSEGGQSAAPSPASWACSPNLRCFTFDRGDISRFPPARPGSLGVMEYYLMDAASLLPVLALGLQPGDIVLDLCAAPGGKTLALLQTGCCRNLAANDLSPSRIARLQKILHSYVPEEIRDGNQVRVTSWDGRKWGELEGDTYDRVLVDVPCTTDRHSLHEEENNIFKRSRKKERQIL Predict reactive species Full Name: NOL1/NOP2/Sun domain family, member 4 Calculated Molecular Weight: 384 aa, 43 kDa Observed Molecular Weight: 40 kDa GenBank Accession Number: BC014441 Gene Symbol: NSUN4 Gene ID (NCBI): 387338 RRID: AB_2935480 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q96CB9 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.