Proteintech, 29903-1-AP, CRELD1 Polyclonal antibody

Size: 20ul / 150ul The CRELD1 (29903-1-AP) by Proteintech is a Polyclonal antibody targeting CRELD1 in WB, IHC, IF/ICC, ELISA applications with reactivity to Human, mouse, rat samples 29903-1-AP targets CRELD1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat samples. Tested Applications Positive WB detected in: HepG2 cells, mouse lung tissue, rat lung tissue Positive IHC detected in: mouse heart tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: U2OS cells Recommended dilution Western Blot (WB): WB : 1:1000-1:6000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information CRELD1 (Cysteine-rich with EGF-like domain protein 1) belongs to matricellular proteins family. The encoded protein has a cysteine-rich with epidermal growth factor-like domain (PubMed: 12137942). Mutations in CRELD1 are the cause of atrioventricular septal defect (PubMed: 24697899). Alternate splicing results in multiple transcript variants. CRELD1 can be detected as 70-75 kDa in mouse lung (PMID: 24697899). Specification Tested Reactivity: Human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag31593 Product name: Recombinant human CRELD1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 218-362 aa of NM_001031717 Sequence: CARCSGPEESNCLQCKKGWALHHLKCVDIDECGTEGANCGADQFCVNTEGSYECRDCAKACLGCMGAGPGRCKKCSPGYQQVGSKCLDVDECETEVCPGENKQCENTEGGYRCICAEGYKQMEGICVKEQIPESAGFFSEMTEDE Predict reactive species Full Name: cysteine-rich with EGF-like domains 1 Calculated Molecular Weight: 45KD Observed Molecular Weight: 45 kDa, 70-75 kDa GenBank Accession Number: NM_001031717 Gene Symbol: CRELD1 Gene ID (NCBI): 78987 RRID: AB_2923619 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q96HD1 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.