Proteintech, 29932-1-AP, RIPK1 Polyclonal antibody

Size: 20ul / 150ul The RIPK1 (29932-1-AP) by Proteintech is a Polyclonal antibody targeting RIPK1 in WB, ELISA applications with reactivity to human samples 29932-1-AP targets RIPK1 in WB, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: K-562 cells, HepG2 cells, L02 cells, MCF-7 cells, MDA-MB-231 cells Recommended dilution Western Blot (WB): WB : 1:1000-1:8000 Background Information RIPK1(Receptor-interacting serine/threonine-protein kinase 1) is primarily involved in mediating TNF-R1-induced cell activation, apoptosis, and necroptosis and belongs to a novel class of kinases that function in cell survival and cell death mechanisms(PMID:22685397 ). It has 2 isoforms produced by alternative splicing. It also can exist as a protein with a lower molecular weight of about 25 kDa and 45 kDa in HAEC and HUVEC(PMID:22685397). Specification Tested Reactivity: human Cited Reactivity: human, pig Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag31950 Product name: Recombinant human RIPK1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 465-671 aa of NM_003804 Sequence: NNGLYSSHGFGTRPLDPGTAGPRVWYRPIPSHMPSLHNIPVPETNYLGNTPTMPFSSLPPTDESIKYTIYNSTGIQIGAYNYMEIGGTSSSLLDSTNTNFKEEPAAKYQAIFDNTTSLTDKHLDPIRENLGKHWKNCARKLGFTQSQIDEIDHDYERDGLKEKVYQMLQKWVMREGIKGATVGKLAQALHQCSRIDLLSSLIYVSQN Predict reactive species Full Name: receptor (TNFRSF)-interacting serine-threonine kinase 1 Calculated Molecular Weight: 76KD Observed Molecular Weight: 70-80 kDa GenBank Accession Number: NM_003804 Gene Symbol: RIPK1 Gene ID (NCBI): 8737 RRID: AB_3086193 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q13546 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.