Proteintech, 30005-1-AP, Phospho-ULK1 (Ser467) Polyclonal antibody

Size: 20ul / 100ul The Phospho-ULK1 (Ser467) (30005-1-AP) by Proteintech is a Polyclonal antibody targeting Phospho-ULK1 (Ser467) in WB, ELISA applications with reactivity to Human, Mouse samples 30005-1-AP targets Phospho-ULK1 (Ser467) in WB, ELISA applications and shows reactivity with Human, Mouse samples. Tested Applications Positive WB detected in: λ phosphatase treated NIH/3T3 cells Recommended dilution Western Blot (WB): WB : 1:500-1:1000 Background Information Unc-51-like-kinase 1 (ULK1) is a target of both the mechanistic target of rapamycin (mTOR) and AMP activated protein kinase (AMPK), whose role is to facilitate the initiation of autophagy in response to starvation. ULK1 is phosphorylated on serine 638 and 758 sites by mTOR in nutrient-rich conditions, inhibiting ULK1 activation by disrupting its binding to AMPK. Upon glucose starvation, dissociation of mTOR from ULK1 and phosphorylation by AMPK leads to the activation of ULK1 activity. AMPK increases ULK1 activity by directly phosphorylating Ser467, Ser555, Thr574, and Ser637 at least four sites, which increases the recruitment of autophagy-relevant proteins (ATG proteins) to the membrane domains which affects autophagy at the initiation stage. S467, S556, and T574 are sites responsible for regulating mitochondrial homeostasis during starvation. (PMID: 30517873, PMID: 21258367) Specification Tested Reactivity: Human, Mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: unc-51-like kinase 1 (C. elegans) Observed Molecular Weight: 113-130 kDa GenBank Accession Number: NM_003565 Gene Symbol: ULK1 Gene ID (NCBI): 8408 RRID: AB_3085348 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: O75385 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.