Proteintech, 30204-1-AP, COL5A3 Polyclonal antibody

Size: 20ul / 150ul The COL5A3 (30204-1-AP) by Proteintech is a Polyclonal antibody targeting COL5A3 in WB, IF/ICC, ELISA applications with reactivity to human samples 30204-1-AP targets COL5A3 in WB, IF/ICC, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: A549 cells, HEK-293T cells Positive IF/ICC detected in: HUVEC cells Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information COL5A3 (Collagen type V alpha 3) belongs to the fibrillar collagen family. Type V collagen (COLV) is a member of a group I collagen (fibrillar forming collagen) and has three varieties of α chains: α1 (COL5A1), α2 (COL5A2), and α3 (COL5A3). COLV is a minor connective tissue component of nearly ubiquitous distribution. It binds to DNA, heparan sulfate, thrombospondin, heparin, and insulin. This antibody is specific to COL5A3. Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag30705 Product name: Recombinant human COL5A3 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 238-380 aa of NM_015719 Sequence: EPETPRPRRKGKGKGRKKGRGRKGKGRKKNKEIWTSSPPPDSAENQTSTDIPKTETPAPNLPPTPTPLVVTSTVTTGLNATILERSLDPDSGTELGTLETKAAREDEEGDDSTMGPDFRAAEYPSRTQFQIFPGAGEKGAKGE Predict reactive species Full Name: collagen, type V, alpha 3 Calculated Molecular Weight: 172 kDa Observed Molecular Weight: 145 kDa GenBank Accession Number: NM_015719 Gene Symbol: COL5A3 Gene ID (NCBI): 50509 RRID: AB_3669706 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P25940 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.