Proteintech, 30210-1-AP, CEP162 Polyclonal antibody

Size: 20ul / 150ul The CEP162 (30210-1-AP) by Proteintech is a Polyclonal antibody targeting CEP162 in WB, IHC, ELISA applications with reactivity to Human samples 30210-1-AP targets CEP162 in WB, IHC, ELISA applications and shows reactivity with Human samples. Tested Applications Positive WB detected in: HEK-293 cells, HeLa cells Positive IHC detected in: human liver tissue, human placenta tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information CEP162, also known as KIAA1009 and QN1, was found in organisms ranging from trypanosomes to vertebrates. A role for CEP162, involved in required to promote assembly of the transition zone in primary cilia. CEP162 acts by specifically recognizing and binding the axonemal microtubule. CEP162 localizes to the distal ends of centrioles before ciliogenesis and directly binds to axonemal microtubule, thereby promoting and restricting transition zone formation specifically at the cilia base. Specification Tested Reactivity: Human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag32953 Product name: Recombinant human CEP162 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 241-438 aa of NM_001286206.1 Sequence: VLLDSLDSVAEVNLDEQDKITPKPRCLPEMTENEMTGTGVSYGQSSSDVEALHQAYCHIAHSLGDEDKQKIESNTVEDIKSSVKGHPQENEENSKNISTMESDLPTVEELMKPIRIDSFGISGFDLQPVSSEKVAERKETEFFSSLPLKMNPNILSQDSQHVNLFFDKNDENVILQKTTNESMENSCPQVTEVTATEE Predict reactive species Full Name: KIAA1009 Calculated Molecular Weight: 162 kDa Observed Molecular Weight: 180 kDa GenBank Accession Number: NM_001286206.1 Gene Symbol: CEP162 Gene ID (NCBI): 22832 RRID: AB_2935531 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q5TB80 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.