Proteintech, 30332-1-AP, ARL13B Polyclonal antibody

Size: 20ul / 150ul The ARL13B (30332-1-AP) by Proteintech is a Polyclonal antibody targeting ARL13B in WB, IF/ICC, IP, ELISA applications with reactivity to human samples 30332-1-AP targets ARL13B in WB, IF/ICC, IP, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: HEK-293 cells, HeLa cells, HepG2 cells, K-562 cells, L02 cells Positive IP detected in: L02 cells Positive IF/ICC detected in: MDCK cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Background Information ARL13B, also named as ARL2L1, is a small ciliary G protein of the Ras superfamily. Localized in the cilia, it is required for cilium biogenesis and sonic hedgehog signaling. Defects in ARL13B are the cause of Joubert syndrome (JS) which is an autosomal recessive disorder characterized by a distinctive cerebellar malformation (PMID: 19906870).This antibody detects two specific bands at 60 kDa and 48 kDa. Arl13b is predicted to be a 48 kDa protein, and the 60 kDa band is likely to represent a modified form of Arl13b. ARL13B can be used to mark the cilia (PMID:22072986). The second antibody is used against guinea pigs. Specification Tested Reactivity: human Host / Isotype: Guinea Pig / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag12015 Product name: Recombinant human ARL13B protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-321 aa of BC094725 Sequence: MFSLMASCCGWFKRWREPVRLANKQDKEGALGEADVIECLSLEKLVNEHKCLCQIEPCSAISGYGKKIDKSIKKGLYWLLHVIARDFDALNERIQKETTEQRALEEQEKQERAERVRKLREERKQNEQEQAELDGTSGLAELDPEPTNPFQPIASVIIENEGKLEREKKNQKMEKDSDGCHLKHKMEHEQIETQGQVNHNGQKNNEFGLVENYKEALTQQLKNEDETDRPSLESANGKKKTKKLRMKRNHRVEPLNIDDCAPESPTPPPPPPPVGWGTPKVTRLPKLEPLGETHHNDFYRKPLPPLAVPQRPNSDAHDVIS Predict reactive species Full Name: ADP-ribosylation factor-like 13B Calculated Molecular Weight: 48 kDa Observed Molecular Weight: 48-55 kDa GenBank Accession Number: BC094725 Gene Symbol: ARL13B Gene ID (NCBI): 200894 RRID: AB_3086296 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q3SXY8 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.