Proteintech, 30376-1-AP, Phospho-RB1 (Ser807/811) Polyclonal antibody

Size: 20ul / 100ul Phospho-RB1 (Ser807/811) Polyclonal Antibody for WB, ELISA 30376-1-AP targets Phospho-RB1 (Ser807/811) in WB, ELISA applications and shows reactivity with Human samples. Tested Applications Positive WB detected in: Calyculin A treated Jurkat cells, Jurkat cells Recommended dilution Western Blot (WB): WB : 1:2000-1:10000 Background Information RB1, also named as pp110, pRb and p105 Rb, belongs to the retinoblastoma protein (RB) family. Phosphorylation and dephosphorylation of RB1 is well known to be a key regulator in cell cycle progression in cancer cells. Phosphorylation of RB1 at multiple S/T residues, first by cyclin D-dependent kinases and subsequently by cyclin E-CDK2 in later G1 phase, completely inactivates RB1, enabling the release of E2F from RB1 complexes and activation of E2F-mediated transcription. Monomethylation at Lys810 by SMYD2 enhances phosphorylation at Ser807 and Ser811, and promotes cell cycle progression. (PMID: 22787429, PMID: 21115810, PMID: 32344731) Specification Tested Reactivity: Human Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: retinoblastoma 1 Calculated Molecular Weight: 928 aa, 106 kDa Observed Molecular Weight: 110 kDa GenBank Accession Number: BC039060 Gene Symbol: RB1 Gene ID (NCBI): 5925 RRID: AB_3085351 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P06400 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.