Proteintech, 30754-1-AP, IL-17A Polyclonal antibody

Size: 20ul / 150ul The IL-17A (30754-1-AP) by Proteintech is a Polyclonal antibody targeting IL-17A in WB, ELISA applications with reactivity to mouse samples 30754-1-AP targets IL-17A in WB, ELISA applications and shows reactivity with mouse samples. Tested Applications Positive WB detected in: PMA, Ionomycin and Brefeldin A treated EL-4 cells Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Background Information IL17A, also named as IL-17, is a proinflammatory cytokine. IL-17, synthesized only by memory T cells and natural killer cells, has pleiotropic effects, mainly in the recruitment and activation of neutrophils. This cytokine regulates the activities of NF-kappaB and mitogen-activated protein kinases. This cytokine can stimulate the expression of IL6 and cyclooxygenase-2 (PTGS2/COX-2), as well as enhance the production of nitric oxide (NO). High levels of this cytokine are associated with several chronic inflammatory diseases including rheumatoid arthritis, psoriasis and multiple sclerosis. The IL-17 receptor is a type I transmembrane protein, that is widely expressed on epithelial cells, fibroblasts, B and T cells, and monocytic cells. In psoriatic skin lesions, both Th17 cells and their downstream effector molecules, e.g. IL-17 and IL-22, are highly increased. Specification Tested Reactivity: mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Recombinant protein Predict reactive species Full Name: interleukin 17A Calculated Molecular Weight: 17 kDa Observed Molecular Weight: 14 kDa, 17 kDa GenBank Accession Number: NM-010552 Gene Symbol: Il17a Gene ID (NCBI): 16171 RRID: AB_3086409 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q62386 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.