Proteintech, 30986-1-AP, CLUH Polyclonal antibody

Size: 20ul / 150ul The CLUH (30986-1-AP) by Proteintech is a Polyclonal antibody targeting CLUH in WB, IHC, IF/ICC, ELISA applications with reactivity to human, mouse, monkey samples 30986-1-AP targets CLUH in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, monkey samples. Tested Applications Positive WB detected in: HeLa cells, mouse heart tissue, mouse kidney tissue, mouse brain tissue, rat brain tissue, rat kidney tissue Positive IHC detected in: mouse brain tissue, mouse kidney tissue, mouse liver tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: COS-7 cells Recommended dilution Western Blot (WB): WB : 1:1000-1:4000 Immunohistochemistry (IHC): IHC : 1:250-1:1000 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information Clustered mitochondria homolog (CLUH) is a conserved RNA-binding protein, that binds transcripts encoding proteins involved in the respiratory chain, the tricarboxylic acid (TCA) cycle, and other mitochondrial metabolic pathways. Upon loss of CLUH, target mRNAs are subjected to faster decay, and their respective proteins are decreased in abundance. This leads to alterations in mitochondrial distribution (mitochondrial clustering as the gene name alludes to) mitochondrial cristae integrity, respiratory defects, loss of mtDNA, and decreased activity of TCA cycle enzymes. (PMID: 35559794) Specification Tested Reactivity: human, mouse, monkey Cited Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag34275 Product name: Recombinant human CLUH protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1120-1309 aa of BC156940 Sequence: YDLSLRFLENALAVSTKYHGPKALKVALSHHLVARVYESKAEFRSALQHEKEGYTIYKTQLGEDHEKTKESSEYLKCLTQQAVALQRTMNEIYRNGSSANIPPLKFTAPSMASVLEQLNVINGILFIPLSQKDLENLKAEVARRHQLQEASRNRDRAEEPMATEPAPAGAPGDLGSQPPAAKDPSPSVQG Predict reactive species Full Name: KIAA0664 Observed Molecular Weight: 147-150 kDa GenBank Accession Number: BC156940 Gene Symbol: CLUH Gene ID (NCBI): 23277 RRID: AB_3669806 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity Purification UNIPROT ID: O75153 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.