Proteintech, 31041-1-AP, MED12L Polyclonal antibody

Size: 20ul / 150ul The MED12L (31041-1-AP) by Proteintech is a Polyclonal antibody targeting MED12L in WB, IF/ICC, ELISA applications with reactivity to human samples 31041-1-AP targets MED12L in WB, IF/ICC, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: HeLa cells, HepG2 cells Positive IF/ICC detected in: U2OS cells Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600 Background Information MED12L, also named as KIAA1635, TNRC11L, TRALP and TRALPUSH, belongs to the Mediator complex subunit 12 family. MED12L may be a component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. MED12L is a mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. MED12L is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag34636 Product name: Recombinant human MED12L protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 627-706 aa of NM_053002 Sequence: LSVTASTRPRSPVGENADEHYSKDHDVKMEIFSPMPGESCENANTSLGRRMSVNCEKLVKREKPRELIFPSNYDLLRHLQ Predict reactive species Full Name: mediator complex subunit 12-like Calculated Molecular Weight: 240 kDa Observed Molecular Weight: 260 kDa GenBank Accession Number: NM_053002 Gene Symbol: MED12L Gene ID (NCBI): 116931 RRID: AB_3669826 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q86YW9 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.