Proteintech, 31232-1-AP, RTTN Polyclonal antibody

Size: 20ul / 150ul The RTTN (31232-1-AP) by Proteintech is a Polyclonal antibody targeting RTTN in WB, ELISA applications with reactivity to human samples 31232-1-AP targets RTTN in WB, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: A549 cells, HeLa cells, Jurkat cells, PC-3 cells Recommended dilution Western Blot (WB): WB : 1:500-1:1000 Background Information RTTN, also known as Rotatin, is an evolutionarily conserved protein that is associated with the centrosome and plays a crucial role in centriole biogenesis. It is found in many organisms and was initially identified in a mutant mouse that exhibited defects in axial rotation and left-right specification. RTTN is required for proper mitotic progression, the maintenance of spindle pole integrity, and correct spindle positioning. Loss of RTTN can lead to mitotic defects, including G2/M phase arrest and increased aneuploidy cells. Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag34960 Product name: Recombinant human RTTN protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1417-1530 aa of NM_173630 Sequence: TVVNILLDQSECSMVRREAAFILQNLLVIPMPTEIIKDYTWQGPCVHDEDSGLSLIGKPALQALLYHCHFYEHLNQMVKHCYLGRCMFDLNFSAFDRNSESNDLNGLDDSFKFW Predict reactive species Full Name: rotatin Calculated Molecular Weight: 249 kDa Observed Molecular Weight: 246 kDa GenBank Accession Number: NM_173630 Gene Symbol: RTTN Gene ID (NCBI): 25914 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity Purification UNIPROT ID: Q86VV8 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.