Proteintech, 31425-1-AP, CORO7 Polyclonal antibody

Size: 20ul / 150ul The CORO7 (31425-1-AP) by Proteintech is a Polyclonal antibody targeting CORO7 in WB, IHC, IP, ELISA applications with reactivity to human samples 31425-1-AP targets CORO7 in WB, IHC, IP, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: HEK-293T cells, HeLa cells, Jurkat cells, MDA-MB-231 cells Positive IP detected in: HeLa cells Positive IHC detected in: human ovary cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:1000-1:4000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information CORO7 also known as CRN7 belongs to the WD repeat coronin family, and functions as an F-actin regulator directing anterograde Golgi to endosome transport(PMID: 15327992). Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag35016 Product name: Recombinant human CORO7 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 750-925 aa of NM_024535 Sequence: KGDTRVFLYELLPESPFFLECNSFTSPDPHKGLVLLPKTECDVREVELMRCLRLRQSSLEPVAFRLPRVRKEFFQDDVFPDTAVIWEPVLSAEAWLQGANGQPWLLSLQPPDMSPVSQAPREAPARRAPSSAQYLEEKSDQQKKEELLNAMVAKLGNREDPLPQDSFEGVDEDEWD Predict reactive species Full Name: coronin 7 Calculated Molecular Weight: 101 kDa Observed Molecular Weight: 100 kDa GenBank Accession Number: NM_024535 Gene Symbol: CORO7 Gene ID (NCBI): 79585 RRID: AB_3669976 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity Purification UNIPROT ID: P57737 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.