Proteintech, 31446-1-AP, CD40 Polyclonal antibody

Size: 20ul / 150ul The CD40 (31446-1-AP) by Proteintech is a Polyclonal antibody targeting CD40 in WB, ELISA applications with reactivity to mouse samples 31446-1-AP targets CD40 in WB, ELISA applications and shows reactivity with mouse samples. Tested Applications Positive WB detected in: J774A.1 cells, RAW 264.7 cells Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Background Information CD40, also known as TNFRSF5 or Bp50, is a member of the tumor necrosis factor (TNF) receptor (TNFR) family. It is expressed on a wide range of cell types including B cells, macrophages, and dendritic cells (PMID: 19426221). CD40 acts as a receptor for TNFSF5/CD40LG, which is expressed on activated T cells. This interaction is essential for B cell proliferation, expression of activation markers, immunoglobulin production, and isotype switching (PMID: 8809473). This interaction is also crucial for the formation of memory B cells and germinal centers, and signaling through CD40 prevents apoptosis of germinal center B cells. Specification Tested Reactivity: mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Recombinant protein Predict reactive species Full Name: CD40 antigen Calculated Molecular Weight: 32 kDa Observed Molecular Weight: 38 kDa GenBank Accession Number: NM_011611.2 Gene Symbol: Cd40 Gene ID (NCBI): 21939 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity Purification UNIPROT ID: P27512-1 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.