Proteintech, 31452-1-AP, Lipocalin-2/NGAL Polyclonal antibody

Size: 20ul / 150ul The Lipocalin-2/NGAL (31452-1-AP) by Proteintech is a Polyclonal antibody targeting Lipocalin-2/NGAL in WB, IHC, ELISA applications with reactivity to rat samples 31452-1-AP targets Lipocalin-2/NGAL in WB, IHC, ELISA applications and shows reactivity with rat samples. Tested Applications Positive WB detected in: rat lung tissue, rat spleen tissue, rat testis tissue Positive IHC detected in: rat spleen tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunohistochemistry (IHC): IHC : 1:200-1:800 Background Information Neutrophil gelatinase-associated lipocalin (NGAL, also known as lipocalin-2, siderocalin, or LCN2) is a small molecule of almost 25 kDa that belongs to the well-defined superfamily of proteins called lipocalins. NGAL was initially found in activated neutrophils, under its role as an innate antibacterial factor. However, it subsequently was shown that many other types of cells, including in the kidney tubule, may produce NGAL in response to various injuries. NGAL may become one of the most promising next-generation biomarkers in clinical nephrology and beyond. This antibody recognizes rat Lipocalin-2/NGAL. Specification Tested Reactivity: rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Recombinant protein Predict reactive species Full Name: lipocalin 2 Calculated Molecular Weight: 22kDa Observed Molecular Weight: 25 kDa Gene Symbol: Lipocalin-2/NGAL Gene ID (NCBI): 170496 RRID: AB_3669991 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity Purification UNIPROT ID: P30152 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.