Proteintech, 31463-1-AP, HSD17B12 Polyclonal antibody

Size: 20ul / 150ul The HSD17B12 (31463-1-AP) by Proteintech is a Polyclonal antibody targeting HSD17B12 in WB, IHC, ELISA applications with reactivity to human samples 31463-1-AP targets HSD17B12 in WB, IHC, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: HT-29 cells, HuH-7 cells, MDA-MB-231 cells Positive IHC detected in: human stomach cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:1000-1:6000 Immunohistochemistry (IHC): IHC : 1:300-1:1200 Background Information HSD17B12 (17β-hydroxysteroid dehydrogenase 12) is an important protein belonging to the hydroxysteroid dehydrogenase family, which is mainly involved in the metabolism of steroids and fatty acids in the body. It has diverse functions in cells and is involved in various biochemical reactions, especially playing a key role in sex hormone synthesis and fatty acid elongation. HSD17B12 can convert estrone into estradiol in ovarian tissue. This enzyme is also involved in fatty acid elongation. HSD17B12 is expressed in a variety of tissues, especially in the brain tissue and the digestive system. In disease states, such as colorectal cancer (CRC), the expression level of HSD17B12 is significantly increased, suggesting that it may play a role in the occurrence and development of tumors. Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag34833 Product name: Recombinant human HSD17B12 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 34-312 aa of BC012043 Sequence: LRVWGVGNEAGVGPGLGEWAVVTGSTDGIGKSYAEELAKHGMKVVLISRSKDKLDQVSSEIKEKFKVETRTIAVDFASEDIYDKIKTGLAGLEIGILVNNVGMSYEYPEYFLDVPDLDNVIKKMININILSVCKMTQLVLPGMVERSKGAILNISSGSGMLPVPLLTIYSATKTFVDFFSQCLHEEYRSKGVFVQSVLPYFVATKLAKIRKPTLDKPSPETFVKSAIKTVGLQSRTNGYLIHALMGSIISNLPSWIYLKIVMNMNKSTRAHYLKKTKKN Predict reactive species Full Name: hydroxysteroid (17-beta) dehydrogenase 12 Calculated Molecular Weight: 312 aa, 34 kDa Observed Molecular Weight: ~34 kDa GenBank Accession Number: BC012043 Gene Symbol: HSD17B12 Gene ID (NCBI): 51144 RRID: AB_3669997 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity Purification UNIPROT ID: Q53GQ0 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.