Proteintech, 31518-1-AP, ACE2 Polyclonal antibody

Size: 20ul / 150ul The ACE2 (31518-1-AP) by Proteintech is a Polyclonal antibody targeting ACE2 in WB, IHC, ELISA applications with reactivity to human, mouse samples 31518-1-AP targets ACE2 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: mouse kidney tissue, mouse small intestine tissue Positive IHC detected in: mouse testis tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:1000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information Angiotensin-converting enzyme 2 (ACE2), a homolog of angiotensin-converting enzyme 1 (ACE1), was identified in 2000. ACE2 provides protection against several chronic diseases, including cardiovascular diseases, lung injury, and diabetes. Besides chronic diseases, ACE2 plays a crucial role in severe acute respiratory syndrome coronavirus (SARS-CoV) infection and severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection (hereafter named COVID-19). ACE2 is the host receptor for SARS-CoV and SARS-CoV-2. (PMID: 37608279) Specification Tested Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Eg0074 Product name: Recombinant Human ACE2 protein (His Tag) Source: mammalian cells -derived, pHZ-KIsec Tag: N-6*His Domain: 18-740 aa of BC048094 Sequence: QSTIEEQAKTFLDKFNHEAEDLFYQSSLASWNYNTNITEENVQNMNNAGDKWSAFLKEQSTLAQMYPLQEIQNLTVKLQLQALQQNGSSVLSEDKSKRLNTILNTMSTIYSTGKVCNPDNPQECLLLEPGLNEIMANSLDYNERLWAWESWRSEVGKQLRPLYEEYVVLKNEMARANHYEDYGDYWRGDYEVNGVDGYDYSRGQLIEDVEHTFEEIKPLYEHLHAYVRAKLMNAYPSYISPIGCLPAHLLGDMWGRFWTNLYSLTVPFGQKPNIDVTDAMVDQAWDAQRIFKEAEKFFVSVGLPNMTQGFWENSMLTDPGNVQKAVCHPTAWDLGKGDFRILMCTKVTMDDFLTAHHEMGHIQYDMAYAAQPFLLRNGANEGFHEAVGEIMSLSAATPKHLKSIGLLSPDFQEDNETEINFLLKQALTIVGTLPFTYMLEKWRWMVFKGEIPKDQWMKKWWEMKREIVGVVEPVPHDETYCDPASLFHVSNDYSFIRYYTRTLYQFQFQEALCQAAKHEGPLHKCDISNSTEAGQKLFNMLRLGKSEPWTLALENVVGAKNMNVRPLLNYFEPLFTWLKDQNKNSFVGWSTDWSPYADQSIKVRISLKSALGDKAYEWNDNEMYLFRSSVAYAMRQYFLKVKNQMILFGEEDVRVANLKPRISFNFFVTAPKNVSDIIPRTEVEKAIRMSRSRINDAFRLNDNSLEFLGIQPTLGPPNQPPVS Predict reactive species Full Name: angiotensin I converting enzyme (peptidyl-dipeptidase A) 2 Calculated Molecular Weight: 805 aa, 92 kDa Observed Molecular Weight: 120 kDa GenBank Accession Number: BC048094 Gene Symbol: ACE2 Gene ID (NCBI): 59272 RRID: AB_3670016 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity Purification UNIPROT ID: Q9BYF1 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.