Proteintech, 31678-1-AP, RAD51C Polyclonal antibody

Size: 20ul / 150ul The RAD51C (31678-1-AP) by Proteintech is a Polyclonal antibody targeting RAD51C in WB, IHC, ELISA applications with reactivity to human, mouse samples 31678-1-AP targets RAD51C in WB, IHC, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: HEK-293T cells, HeLa cells, MDA-MB-231 cells, mouse testis tissue Positive IHC detected in: mouse testis tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:1000-1:5000 Immunohistochemistry (IHC): IHC : 1:250-1:1000 Background Information RAD51C (DNA repair protein RAD51 homolog 3) is essential for the homologous recombination (HR) pathway of DNA repair and is involved in the homologous recombination repair (HRR) pathway of double-stranded DNA breaks arising during DNA replication or induced by DNA-damaging agents (PMID: 14716019; 16215984; 16395335). Specification Tested Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag35070 Product name: Recombinant human RAD51C protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-376 aa of BC107753 Sequence: MRGKTFRFEMQRDLVSFPLSPAVRVKLVSAGFQTAEELLEVKPSELSKEVGISKAEALETLQIIRRECLTNKPRYAGTSESHKKCTALELLEQEHTQGFIITFCSALDDILGGGVPLMKTTEICGAPGVGKTQLCMQLAVDVQIPECFGGVAGEAVFIDTEGSFMVDRVVDLATACIQHLQLIAEKHKGEEHRKALEDFTLDNILSHIYYFRCRDYTELLAQVYLLPDFLSEHSKVRLVIVDGIAFPFRHDLDDLSLRTRLLNGLAQQMISLANNHRLAVILTNQMTTKIDRNQALLVPALGESWGHAATIRLIFHWDRKQRLATLYKSPSQKECTVLFQIKPQGFRDTVVTSACSLQTEGSLSTRKRSRDPEEEL Predict reactive species Full Name: RAD51 homolog C (S. cerevisiae) Calculated Molecular Weight: 376 aa, 42 kDa Observed Molecular Weight: 40-42 kDa GenBank Accession Number: BC107753 Gene Symbol: RAD51C Gene ID (NCBI): 5889 RRID: AB_3670072 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity Purification UNIPROT ID: O43502 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.