Proteintech, 32089-1-AP, CD2AP Polyclonal antibody

Size: 20ul / 150ul The CD2AP (32089-1-AP) by Proteintech is a Polyclonal antibody targeting CD2AP in WB, IF/ICC, ELISA applications with reactivity to human, mouse, rat samples 32089-1-AP targets CD2AP in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: A431 cells, mouse testis tissue, Daudi cells, Jurkat cells, K-562 cells, rat testis tissue Positive IF/ICC detected in: HepG2 cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information CD2 associated protein (CD2AP, also known as CMS) is a scaffolding molecule that acts as an adapter protein between membrane proteins and the actin cytoskeleton (PMID: 10339567). CD2AP also plays a role in epithelial cell junction formation and affects the pathogenesis of Alzheimer's disease (PMID: 22891260; 31440393). Its mutations are associated with sporadic nephrotic syndrome and focal segmental glomerulosclerosis (FSGS) (PMID: 17713465; 19131354). Specification Tested Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag36919 Product name: Recombinant human CD2AP protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 400-639 aa of NM_012120.2 Sequence: NLLRSSGTVYPKRPEKPVPPPPPIAKINGEVSSISSKFETEPVSKLKLDSEQLPLRPKSVDFDSLTVRTSKETDVVNFDDIASSENLLHLTANRPKMPGRRLPGRFNGGHSPTHSPEKILKLPKEEDSANLKPSELKKDTCYSPKPSVYLSTPSSASKANTTAFLTPLEIKAKVETDDVKKNSLDELRAQIIELLCIVEALKKDHGKELEKLRKDLEEEKTMRSNLEMEIEKLKKAVLSS Predict reactive species Full Name: CD2-associated protein Calculated Molecular Weight: 71 kDa，639aa Observed Molecular Weight: 71 kDa GenBank Accession Number: NM_012120.2 Gene Symbol: CD2AP Gene ID (NCBI): 23607 RRID: AB_3670188 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity Purification UNIPROT ID: Q9Y5K6 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.