Proteintech, 32180-1-AP, IL-23 Polyclonal antibody

Size: 20ul / 150ul The IL-23 (32180-1-AP) by Proteintech is a Polyclonal antibody targeting IL-23 in WB, IF/ICC, ELISA applications with reactivity to human samples 32180-1-AP targets IL-23 in WB, IF/ICC, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: Jurkat cells, SW480 cells, U266 cells Positive IF/ICC detected in: Raji cells Recommended dilution Western Blot (WB): WB : 1:500-1:1000 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information IL-12 and IL-23 are heterodimeric cytokines with a common p40 subunit (PMID: 11114383). IL-12 is composed of the IL-12 p40 subunit linked to the IL-12 p35 subunit, and the heterodimer signals through the IL-12 receptor (IL-12R), which comprises the IL-12Rβ1 and IL-12Rβ2 subunits. IL-23 is composed of the IL-23 p19 subunit and the IL-12 p40 (IL-12/23p40) subunit, which signals through IL-23R and IL-12Rβ1 (PMID: 11114383; 26121196 ). The importance of IL-23 has been confirmed by clinical data, as monoclonal antibodies (MABs) targeting the p40 subunit (shared by IL-23 and IL-12), as well as p19 (specific for IL-23) were effective in therapy for psoriasis and psoriatic arthritis (PMID: 25754330; 18486740). This antibody can recognize the IL12B monomer (37 kDa) and the heterodimer (IL12B+IL23A, 54 kDa). Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Eg1304 Product name: Recombinant Human IL-23 protein (His Tag) Source: mammalian cells -derived, pHZ-KIsec-C-6*HIS Tag: C-6*HIS Domain: 23-328 aa of Sequence: IWELKKDVYVVELDWYPDAPGEMVVLTCDTPEEDGITWTLDQSSEVLGSGKTLTIQVKEFGDAGQYTCHKGGEVLSHSLLLLHKKEDGIWSTDILKDQKEPKNKTFLRCEAKNYSGRFTCWWLTTISTDLTFSVKSSRGSSDPQGVTCGAATLSAERVRGDNKEYEYSVECQEDSACPAAEESLPIEVMVDAVHKLKYENYTSSFFIRDIIKPDPPKNLQLKPLKNSRQVEVSWEYPDTWSTPHSYFSLTFCVQVQGKSKREKKDRVFTDKTSATVICRKNASISVRAQDRYYSSSWSEWASVPCSGGGGSGGGGSGGGGSRAVPGGSSPAWTQCQQLSQKLCTLAWSAHPLVGHMDLREEGDEETTNDVPHIQCGDGCDPQGLRDNSQFCLQRIHQGLIFYEKLLGSDIFTGEPSLLPDSPVGQLHASLLGLSQLLQPEGHHWETQQIPSLSPSQPWQRLLLRFKILRSLQAFVAVAARVFAHGAATLSP Predict reactive species Full Name: IL-23 Calculated Molecular Weight: 54 kDa, 37 kDa, 21 kDa Observed Molecular Weight: 54 kDa, 37 kDa RRID: AB_3670216 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity Purification UNIPROT ID: P29460(IL12B)+linker+Q9NPF7(IL23A) Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.