Proteintech, 32452-1-AP, BRD1/BRL Polyclonal antibody

Size: 20ul / 150ul The BRD1/BRL (32452-1-AP) by Proteintech is a Polyclonal antibody targeting BRD1/BRL in WB, IP, ELISA applications with reactivity to human samples 32452-1-AP targets BRD1/BRL in WB, IP, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: Jurkat cells, SH-SY5Y cells, SK-N-SH cells Positive IP detected in: Jurkat cells Recommended dilution Western Blot (WB): WB : 1:500-1:3000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Background Information BRD1, also known as BRL and BRPF2, plays a key role in chromatin recruitment to modulate histone acetylation and consequently gene transcription. It interacts with a range of epigenetic modifiers, including histone acetyltransferases KAT5 and KAT7, as well as the histone-lysine N-methyltransferase KMT5B (PMID: 29453316, 38383216). It has 2 isoforms with the molecular mass of 133 kDa and 120 kDa. Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag37530 Product name: Recombinant human BRD1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 918-1070 aa of BC047508 Sequence: VLPRLETLLQPRKRSRSTCGDSEVEEESPGKRLDAGLTNGFGGARSEQEPGGGLGRKATPRRRCASESSISSSNSPLCDSSFNAPKCGRGKPALVRRHTLEDRSELISCIENGNYAKAARIAAEVGQSSMWISTDAAASVLEPLKVVWAKCSG Predict reactive species Full Name: bromodomain containing 1 Calculated Molecular Weight: 120 kDa Observed Molecular Weight: 133 kDa GenBank Accession Number: BC047508 Gene Symbol: BRD1 Gene ID (NCBI): 23774 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity Purification UNIPROT ID: O95696 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.