Proteintech, 32468-1-AP, HSPA4L Polyclonal antibody

Size: 20ul / 150ul The HSPA4L (32468-1-AP) by Proteintech is a Polyclonal antibody targeting HSPA4L in WB, IHC, ELISA applications with reactivity to human samples 32468-1-AP targets HSPA4L in WB, IHC, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: HL-60 cells, K-562 cells, RT-4 cells, U-251 cells Positive IHC detected in: human tonsillitis tissue, human lung cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:1000-1:8000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information HSPA4L (Heat shock 70-kDa protein 4-like), also known as APG1 or OSP94, is a member of the heat shock protein 110 family, whose mRNA is strongly expressed in normal human testis and overexpressed in leukemia cells. HSPA4L was also restrictedly expressed in normal tissues and the its product was capable of eliciting a humoral immune response likely induced by its high-level expression in leukemia cells. Up-regulation of the Hspa4l gene in kidney and renal cell lines by osmotic stress suggested that Hspa4l may function to enable the kidney to compensate for osmotic stress present within the kidney (PMID: 16923965, 17588478). Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag38354 Product name: Recombinant human HSPA4L protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 690-839 aa of NM_001317381.1 Sequence: MKYMEHEERPKALNDLGKKIQLVMKVIEAYRNKDERYDHLDPTEMEKVEKCISDAMSWLNSKMNAQNKLSLTQDPVVKVSEIVAKSKELDNFCNPIIYKPKPKAEVPEDKPKANSEHNGPMDGQSGTETKSDSTKDSSQHTKSSGEMEVD* Predict reactive species Full Name: heat shock 70kDa protein 4-like Calculated Molecular Weight: 95kDa，839aa Observed Molecular Weight: 95~100 kDa GenBank Accession Number: NM_001317381.1 Gene Symbol: HSPA4L Gene ID (NCBI): 22824 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity Purification UNIPROT ID: O95757 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.