Proteintech, 32482-1-AP, RNF220 Polyclonal antibody

Size: 20ul / 150ul The RNF220 (32482-1-AP) by Proteintech is a Polyclonal antibody targeting RNF220 in WB, ELISA applications with reactivity to human, mouse, rat samples 32482-1-AP targets RNF220 in WB, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: RT-4 cells, U-251 cells, mouse brain tissue, rat brain tissue Recommended dilution Western Blot (WB): WB : 1:2000-1:12000 Background Information RNF220 is an E3 ubiquitin ligase that plays a crucial role in the ubiquitination process, which targets proteins for degradation by the proteasome. Mutations in RNF220 have been linked to laminopathies, which are characterized by leukodystrophy, ataxia, and deafness. Additionally, RNF220 is involved in the progression of certain cancers, such as acute myeloid leukemia (AML) and colorectal cancer. Studies have shown that RNF220 promotes the proliferation of leukemia cells and reduces the degradation of Cyclin D1 protein through USP22. It also enhances canonical Wnt signaling through USP7-mediated deubiquitination of beta-catenin. (PMID: 32896826) Specification Tested Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag36190 Product name: Recombinant human RNF220 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 253-566 aa of BC034221 Sequence: SKNSLLKDAMAPGTPKSLLLSASIKREGESPTASPHSSATDDLHHSDRYQTFLRVRANRQTRLNARIGKMKRRKQDEGQREGSCMAEDDAVDIEHENNNRFEEYEWCGQKRIRATTLLEGGFRGSGFIMCSGKENPDSDADLDVDGDDTLEYGKPQYTEADVIPCTGEEPGEAKEREALRGAVLNGGPPSTRITPEFSKWASDEMPSTSNGESSKQEAMQKTCKNSDIEKITEDSAVTTFEALKARVRELERQLSRGDRYKCLICMDSYSMPLTSIQCWHVHCEECWLRTLGAKKLCPQCNTITAPGDLRRIYL Predict reactive species Full Name: ring finger protein 220 Observed Molecular Weight: 70 kDa GenBank Accession Number: BC034221 Gene Symbol: RNF220 Gene ID (NCBI): 55182 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity Purification UNIPROT ID: Q5VTB9 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.