Proteintech, 32944-1-AP, GTPBP10 Polyclonal antibody

Size: 20ul / 150ul The GTPBP10 (32944-1-AP) by Proteintech is a Polyclonal antibody targeting GTPBP10 in WB, ELISA applications with reactivity to human, mouse samples 32944-1-AP targets GTPBP10 in WB, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: HEK-293T cells, HeLa cells, RT-4 cells Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Background Information GTPBP10, a member of the conserved Obg family of P-loop small G proteins, is a mitochondrial protein. The absence of GTPBP10 leads to attenuated mtLSU and mtSSU levels and the virtual absence of the 55S monosome, which entirely prevents mitochondrial protein synthesis. GTPBP10 physically interacts with the 16S rRNA, but not with the 12S rRNA, and crosslinks with several mtLSU proteins. Additionally, GTPBP10 is indirectly required for efficient processing of the 12S-16S rRNA precursor transcript, which could explain the mtSSU accumulation defect (PMID: 30321378). Specification Tested Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag37974 Product name: Recombinant human GTPBP10 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-387 aa of BC107714 Sequence: MVHCSCVLFRKYGNFIDKLRLFTRGGSGGMGYPRLGGEGGKGGDVWVVAQNRMTLKQLKDRYPGKRFVAGVGANSKISALKGSKGKDWEIPVPVGISVTDENGKIIGELNKENDRILVAQGGLGGKLLTNFLPLKGQKRIIHLDLKLIADVGLVGFPNAGKSSLLSCVSHAKPAIADYAFTTLKPELGKIMYSDFKQISVADLPGLIEGAHMNKGMGHKFLKHIERTRQLLFVVDISGFQLSSHTQYRTAFETIILLTKELELYKEELQTKPALLAVNKMDLPDAQDKFHELMSQLQNPKDFLHLFEKNMIPERTVEFQHIIPISAVTGEGIEELKNCIRKSLDEQANQENDALHKKQLLNLWISDTMSSTEPPSKHAVTTSKMDII Predict reactive species Full Name: GTP-binding protein 10 (putative) Calculated Molecular Weight: 43, 34, 12 kDa Observed Molecular Weight: 42, 34, 20 kDa GenBank Accession Number: BC107714 Gene Symbol: GTPBP10 Gene ID (NCBI): 85865 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity Purification UNIPROT ID: A4D1E9 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.