Proteintech, 32953-1-AP, PANK4 Polyclonal antibody

Size: 20ul / 150ul The PANK4 (32953-1-AP) by Proteintech is a Polyclonal antibody targeting PANK4 in WB, ELISA applications with reactivity to human, mouse, rat samples 32953-1-AP targets PANK4 in WB, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HEK-293 cells, HeLa cells, mouse skeletal muscle tissue, rat skeletal muscle tissue Recommended dilution Western Blot (WB): WB : 1:500-1:3000 Background Information PANK4 (Pantothenate Kinase 4) is a member of the pantothenate kinase family, which is involved in the biosynthesis of coenzyme A (CoA). However, unlike other members of the family, PANK4 lacks the residues required for kinase activity and instead contains a phosphatase domain (DUF89) that can dephosphorylate 4'-phosphopantetheine, an intermediate in CoA biosynthesis (PMID: 30927326). This suggests that PANK4 may act as a pseudoenzyme, regulating cellular CoA levels by antagonizing CoA production (PMID: 39746949). Specification Tested Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag38232 Product name: Recombinant human PANK4 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-178 aa of NM_018216.2 Sequence: MAECGASGSGSSGDSLDKSITLPPDEIFRNLENAKRFAIDIGGSLTKLAYYSTVQHKVAKVRSFDHSGKDTEREHEPPYEISVQEEITARLHFIKFENTYIEACLDFIKDHLVNTETKVIQATGGGAYKFKDLIEEKLRLKVDKEDVMTCLIKGCNFVLKNIPHEAFVYQKDSDPEFR Predict reactive species Full Name: pantothenate kinase 4 Calculated Molecular Weight: 86 kDa，773aa Observed Molecular Weight: 86 kDa GenBank Accession Number: NM_018216.2 Gene Symbol: PANK4 Gene ID (NCBI): 55229 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity Purification UNIPROT ID: Q9NVE7 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.