Proteintech, 33159-1-AP, BMP-9 Polyclonal antibody

Size: 20ul / 150ul The BMP-9 (33159-1-AP) by Proteintech is a Polyclonal antibody targeting BMP-9 in WB, ELISA applications with reactivity to mouse, rat samples 33159-1-AP targets BMP-9 in WB, ELISA applications and shows reactivity with mouse, rat samples. Tested Applications Positive WB detected in: mouse liver tissue, rat liver tissue Recommended dilution Western Blot (WB): WB : 1:1000-1:6000 Background Information Bone Morphogenetic Protein 9 (BMP9), also known as Growth Differentiation Factor 2 (GDF-2), is a cytokine belonging to the transforming growth factor-β (TGF-β) superfamily. BMP9 is mainly produced by hepatocytes and hepatic stellate cells in the liver and is secreted into the bloodstream, where it acts as a circulating factor involved in various physiological processes. BMP9 is synthesized as a precursor protein (pre-proBMP9) composed of 429 amino acids (aa) that includes a 22 aa signal peptide, a 297 aa prodomain (33 kDa) and a 110 aa mature protein (12.5 kDa). It is then cleaved by serine endoproteases, leading to a short dimeric mature form of 25 kDa, and the prodomain which can remain noncovalently associated with the mature short form, giving a size of about 100 kDa. (PMID:21710321) Specification Tested Reactivity: mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Eg2482 Product name: recombinant mouse Gdf2 protein Source: mammalian cells -derived, V37 Tag: C-rFc Domain: 23-428 aa of NM_019506.4 Sequence: KPLQNWEQASPGENAHSSLGLSGAGEEGVFDLQMFLENMKVDFLRSLNLSGIPSQDKTRAEPPQYMIDLYNRYTTDKSSTPASNIVRSFSVEDAISTAATEDFPFQKHILIFNISIPRHEQITRAELRLYVSCQNDVDSTHGLEGSMVVYDVLEDSETWDQATGTKTFLVSQDIRDEGWETLEVSSAVKRWVRADSTTNKNKLEVTVQSHRESCDTLDISVPPGSKNLPFFVVFSNDRSNGTKETRLELKEMIGHEQETMLVKTAKNAYQVAGESQEEEGLDGYTAVGPLLARRKRSTGASSHCQKTSLRVNFEDIGWDSWIIAPKEYDAYECKGGCFFPLADDVTPTKHAIVQTLVHLKFPTKVGKACCVPTKLSPISILYKDDMGVPTLKYHYEGMSVAECGCR Predict reactive species Full Name: growth differentiation factor 2 Calculated Molecular Weight: 48 kDa Observed Molecular Weight: 33 kDa GenBank Accession Number: NM_019506.4 Gene Symbol: BMP9 Gene ID (NCBI): 12165 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity Purification UNIPROT ID: Q9WV56 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.