Proteintech, 33193-1-AP, PREX1 Polyclonal antibody

Size: 20ul / 150ul The PREX1 (33193-1-AP) by Proteintech is a Polyclonal antibody targeting PREX1 in WB, IF/ICC, ELISA applications with reactivity to human samples 33193-1-AP targets PREX1 in WB, IF/ICC, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: MCF-7 cells Positive IF/ICC detected in: MCF-7 cells Recommended dilution Western Blot (WB): WB : 1:1000-1:8000 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information Phosphatidylinositol 3,4,5-trisphosphate-dependent Rac exchanger 1 protein (PREX1) is a guanine nucleotide exchange factor (GEF) that specifically activates Rac GTPases, particularly Rac1 (PMID: 16243035). PREX1 is activated downstream of G protein-coupled receptors (GPCRs) and receptor tyrosine kinases (RTKs), such as ErbB receptors, and is involved in PI3K/AKT and MAPK signaling pathways (PMID: 28710285; 27358402). It contributes to glucose uptake in adipocytes and promotes tumor cell proliferation, migration, and metastasis in various cancers, including breast, prostate, and melanoma (PMID: 22109529). Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag36649 Product name: Recombinant human PREX1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 700-900 aa of NM_020820.3 Sequence: LLVATKAKEIIKIPDQPDTLCFQIRGAAPPYVYAVGRGSEAMAAGLCAGQCILKVNGSNVMNDGAPEVLEHFQAFRSRREEALGLYQWIYHTHEDAQEARASQEASTEDPSGEQAQEEDQADSAFPLLSLGPRLSLCEDSPMVTLTVDNVHLEHGVVYEYVSTAGVRCHVLEKIVEPRGCFGLTAKILEAFAANDSVFVEN Predict reactive species Full Name: phosphatidylinositol-3,4,5-trisphosphate-dependent Rac exchange factor 1 Calculated Molecular Weight: 186 kDa Observed Molecular Weight: 186 kDa GenBank Accession Number: NM_020820.3 Gene Symbol: PREX1 Gene ID (NCBI): 57580 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity Purification UNIPROT ID: Q8TCU6 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.